REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
Plasma/Urine/Tissue/EVs samples	The Department of Urology of Fudan University Shanghai Cancer Center (FUSCC)	This paper
Antibodies
Calnexin Rabbit mAb	ABclonal	Cat# A4846; RRID: AB_2863359
CD9 Rabbit mAb	ABclonal	Cat# A19027; RRID: AB_2862519
HSP70 Rabbit mAb	ABclonal	Cat# A21180; RRID: AB_2943041
TSG101/VPS23 Rabbit mAb	ABclonal	Cat# A5789; RRID: AB_2863517
Chemicals, peptides, and recombinant proteins
HPLC-grade water	J.T. Baker	Catalog: 4218-03
Phenylmethylsulfonyl fluoride	Amresco	Catalog: M145
Dithiothreitol	Sigma	Catalog: 43815
Iodoacetamide	Sigma	Catalog: V900335
Formic acid	Sigma	Catalog: 507
Trypsin	Promega	Catalog: V528A
Ammonium bicarbonate	J.T. Baker	Catalog: 3003-01
Acetonitrile	J.T. Baker	Catalog: 9829-03
Iodoacetamide	Sigma	Catalog: V900335
Dithiothreitol	Sigma	Catalog: 43815
qEVoriginal	IZON	Catalog: ICO-70
Instrumentation		N/A
Q Exactive HF-X Hybrid		N/A
Quadrupole-Orbitrap	ThermoFisher	Catalog: 726042
Mass Spectrometer		N/A
EASY-nLC 1200 system	ThermoFisher	Catalog: LC140
Vacuum centrifuge	Eppendorf	Catalog: 07-748-15
Deposited data
Proteomic data	This paper	iProx: IPX0005194000
Fudan bladder cancer cohort	Xu et al.2	PMID: 35659036
TCGA cohort	Robertson et al., 201761	PMID: 30096301
The human protein atlas database (version: 21.1, released 2022-05-31)	Human protein atlas (HPA)	https://www.proteinatlas.org/
Software and algorithms
R version v 3.5.1	The R Project for Statistical Computing	https://www.r-project.org/
RStudio (v1.2.5019)	R Studio Team, 2015	https://rstudio.com/products/rstudio/download/
GraphPad Prism (version 8.0.1)	GraphPad	https://www.graphpad.com/scientific-software/prism/
Firmiana	Feng et al.62	https://phenomics.fudan.edu.cn/firmiana/gardener/
Mascot (version 2.4)	Matrix Science	http://www.matrixscience.com/
UniProt human protein database (released on 09-06-2021)	The UniProt Consortium	https://www.uniprot.org/
ConsensusPathDB	Herwig et al., 201663	http://cpdb.molgen.mpg.de/CPDB/