REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SMAD 2/3	Cell Signaling Technology	Cat#8685; RRID: AB_10889933
Phospho-SMAD 2/3	Cell Signaling Technology	Cat#8828; RRID: AB_2631089
β-Tubulin	Fisher	Cat#322600; RRID: AB_2533072
Chemicals, peptides, and recombinant proteins
Dextrose	Caledon Laboratory Chemicals	Cat#3260-1-70
Insulin, Human Recombinant	Fisher Scientific	Cat#12585014
Formalin Buffered 10%	ACP Chemicals	Cat#F6000
TRIzol Reagent	ThermoFisher Scientific	Cat#15596026
Sodium Pyruvate (powder)	Sigma-Aldrich	Cat#P2256
DNase I	Roche	Cat#10104159001
Critical commercial assays
RNeasy Mini Kit	Qiagen	Cat#74106
Alanine Transaminase Microplate Assay Kit	Cohesion Biosciences	Cat#CAK1002
Aspartate Transaminase Microplate Assay Kit	Cohesion Biosciences	Cat#CAK1004
Cholesterol E kit	Fujifilm	Cat#999-02601
Non-esterified fatty acid kit (NEFA-HR(2))	Fujifilm	Cat# 999–34691, 991–34891, 993-35191
Triglyceride Kit	Cayman Chemical	Cat#10010303
Ultra-Sensitive Mouse Insulin ELISA kit	Crystal Chem	Cat#90080
Serum Amyloid A kit	R&D Systems	Cat#MSAA00
Secretory Phospholipase A2 kit	Cayman Chemical	Cat#765001-96
CXCL10 kit	ThermoFisher	Cat#
nCounter Fibrosis Panel	NanoString Technologies	Cat#115000388
Pierce BCA Protein Assay Kit	ThermoFisher Scientific	Cat#23225
Deposited data
Human NAFLD livers with different fibrosis stages	Govaere et al.33	GEO: GSE135251
Whole liver NanoString data superseries	This study	GEO: GSE240424
Whole liver NanoString data commercial fibrosis kit	This study	GEO: GSE240406
Whole liver NanoString data custom kit	This study	GEO: GSE240409
Experimental models: Organisms/strains
Mouse: C57BL/6J	The Jackson Laboratory	Cat#000664; RRID: AB_IMSR_JAX:000664
Software and algorithms
ImageJ	N/A	https://imagej.nih.gov/ij/
nSolver Analysis Software 4.0	NanoString	https://nanostring.com/products/analysis-solutions/nsolver-advanced-analysis-software/
R version 3.6.0 (R Core Team (2022)	R	https://www.r-project.org/
GraphPad Prism version 9.3.1 (350)	GraphPad	https://www.graphpad.com/scientific-software/prism/
Other
Rodent diet with 40% kcal fat, 20% kcal fructose, 0.02% cholesterol	Research Diets	Cat#D19101102
Accu-Chek Inform II system (glucometer)	Roche	N/A
Accu-Chek Guide (test strips)	Roche	N/A
Specific-pathogen free (SPF) Microisolators	The Jackson Laboratory	N/A
Nikon 90i Eclipse	Nikon	N/A