Figure 3.

High PPC downregulates development-related genes and disrupts sugar metabolism in the ovary

(A) RNA-seq timeline. The ovaries in P2 and P4 with the significantly increasing PPC were used for RNA-seq.

(B) Heatmap of sample correlation analysis. T2 and T4 represent TBH in P2 and P4, respectively. W2 and W4 represent WT in P2 and P4, respectively.

(C) Number of DEGs.

(D) Relative expression of genes related to ovarian development (TPM). SLC25A26, mitochondrial SAM transporter gene; 5-HTR, 5-hydroxytryptamine receptor gene; ZIP3, zinc transporter ZIP3 gene; ESRRA, estrogen related receptor gene; GABBR, γ-GABA receptor gene; CA7, carbonic anhydrase 7. The vertical axis shows the ratio of expression changes of different genes with WT as control (log2 Fold change).

(E and E′) Relative expression of Tret1 (TPM) and qRT-PCR validation results (n = 3). Tret1, facilitated trehalose transporter1.

(F) Trehalose content in the P4 ovary (n = 3).

(G) Relative expression of Treh2 (n = 3). Treh2, trehalase2 gene.

(H and I) Glycogen content in the P4 ovary (H) and laid egg (I) (n = 3). sw22934 was used as the reference gene for qRT-PCR.

The data of (E′–I) were mean ± SD, and the significance of the difference in the Student’s t test was ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.