Figure 4.
Plotted standard curves fitted according to various cortisol concentrations
(A) Standard curves obtained by commercial competitive ELISA kit with HRP-cortisol/cortisol solution of 150 μL/50 μL (150:50), 200 μL/25 μL (200:25), and 200 μL/50 μL (200:50), respectively.
(B) Standard curves obtained by the microfluidic system immunoassay with HRP-cortisol/cortisol solution of 150:50, 200:25, and 200:50, respectively. Error bars: standard error of the mean from n = 3 independent measurements.
