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. 2023 Sep 25;42:248. doi: 10.1186/s13046-023-02803-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — CHSY1 facilitates the proliferation, invasion and migration of CRC cells. A, B Three shRNAs (sh1, sh2, and sh3) were designed to silence CHSY1 in CRC cells (HCT116 and LOVO), and validated by qRT-PCR and western blotting. C EdU assay was performed to assess cell proliferation of CRC cells transfected with sh3-CHSY1/sh-NC. D The growth curves of cells were plotted after transfection with sh3-CHSY1/sh-NC based on CCK-8 assay. E Transwell invasion and migration assay were performed to assess invasion and migration of CRC cells transfected with sh3-CHSY1/sh-NC.Scale bar, 100 μm. F Wound Healing assay was performed to assess migration of CRC cells transfected with sh3-CHSY1/sh-NC.Scale bar, 100 μm. G-HThree shRNAs (sh1, sh2, and sh3) were designed to silence CHSY1 in MC38 cells, and validated by qRT-PCR and western blotting. *, P < 0.05; **, P < 0.01; ***, P < 0.001;****, P < 0.0001