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. 2023 Sep 25;42:248. doi: 10.1186/s13046-023-02803-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The anti-tumor effect of knockdown CHSY1 combined with anti-PD1 in vivo. A Schematic diagram of the establishment of a mouse model of CRC liver metastases. B Images of liver metastases in each group. C Analysis of liver metastases tumors in the respective groups. (multiple unpaired t test) (D, E) Immunohistochemistry results of CD8, CD4, Ki67, PD-L1 and PD1 expression in the respective groups. F, G The immunofluorescence was used to detect the expression of CHSY1 and PD-L1 in liver metastases in each group. *, P < 0.05; **, P < 0.01; ***, P < 0.001;****, P < 0.0001.ns indicates no statistical difference