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. Author manuscript; available in PMC: 2023 Sep 25.
Published in final edited form as: Curr Biol. 2022 Jul 20;32(17):3690–3703.e5. doi: 10.1016/j.cub.2022.06.089

Figure 6. In male mice, developmental feeding history affected AMPAR/NMDAR ratio in mesolimbic dopamine neurons in adulthood.

Figure 6.

A, Retrobeads were injected into the NAc core and recordings were made from the VTA in naïve male AL and FI mice at P61-70. B, Example of evoked EPSC traces before and after application of D-AP5. The dual components of AMPAR-mediated and NMDAR-mediated EPSCs were recorded. D-AP5 was applied to isolate AMPAR-mediated currents. C, The AMPAR/NMDAR ratio was significantly reduced in the FI group (n=10) compared to the AL group (n=11). D,E, AMPAR-mediated EPSC(I)-Voltage(V) relationship curve. There was a trend level difference in current at +40mV in the slices from the FI group (treatment: F(1,90)=1.367, p=0.25, voltage: F(4,90)=56.56, p<0.0001, interaction: F(4,90)=1.105, p=0.36, post-hoc Tukey at +40mV: p=0.096). There was no significant difference in rectification index between the AL(n=9) and FI (n=9) groups. F,G, Paired-pulse ratios were not significantly different between groups at 50, 100, and 200 ms intervals. *p<0.05. Data are represented as mean ± SEM.