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. Author manuscript; available in PMC: 2023 Sep 25.
Published in final edited form as: Methods Mol Biol. 2023;2551:561–573. doi: 10.1007/978-1-0716-2597-2_34

Fig. 1.

Fig. 1

ROI identification for neurons coexpressing mApple (red) and iRFP-UBQLN2 (cyan). mApple channel images are thresholded using the “minimum dark” option. FIJI’s “Analyze Particles” tool is then used to superimpose ROIs onto objects larger than the “min_cell_area” specified by the user. These ROIs are saved to FIJI’s ROI manager and overlayed onto the iRFP channel to measure single-cell iRFP-UBQLN2 fluorescence intensity and standard deviation. Red numbers specify cell death. Cyan numbers indicate neurons with UBQLN2 puncta. Scale bar = 100 μm