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. Author manuscript; available in PMC: 2023 Sep 25.
Published in final edited form as: Methods Mol Biol. 2023;2551:561–573. doi: 10.1007/978-1-0716-2597-2_34

Fig. 3.

Fig. 3

Percentage of neurons containing UBQLN2 puncta over time. Using a CVintensity threshold of 0.51 as determined in Subheading 3.7, the percentage of puncta(+) neurons is plotted for neurons expressing iRFP-WT-UBQLN2, iRFP-P506T-UBQLN2 (mutant), and iRFP alone. The absence of puncta in the iRFP alone control shows that the chosen CVintensity threshold produces few false positives