Prion Disease After COVID-19: A Case Report

Abstract

Patient: Male, 62-year-old

Final Diagnosis: COVID 19 infection • Creutzfeldt-Jakob disease • prion disease

Symptoms: Bradykinesia • difficulty in walking • dysphagia • myoclonus • rapidly progressive dementia

Clinical Procedure: —

Specialty: Neurology

Objective:

Unknown etiology

Background:

Prion disease (PrD) is one of the rapidly progressive dementias. It typically requires several diagnostic criteria to fulfill a probable diagnosis, as definite diagnosis is based on isolated brain biopsy. There has been much debate on a possible infectious etiology of PrD. Viral infections are commonly pathologic in most neurodegenerative conditions. In PrD, misfolded proteins can be contagious and act as infective proteins, regardless of the pathologic agent. There is evidence that COVID-19 can result in neurologic manifestations, and neurodegeneration has been reported in the literature. There are several case reports describing parkinsonism after COVID-19, with Parkinson’s disease in particular noted in COVID-19. Few cases of PrD were reported after COVID-19 infection. We identified 1 case of PrD in the setting of COVID-19 at our hospital.

Case Report:

We report the case of a 62-year-old man admitted to Mount Sinai Queens Hospital Center, who presented with rapidly progressive dementia along with difficulty walking and myoclonus. All workup results were negative. He underwent MRI brain, but results were not revealing. Due to the high clinical suspicion, CSF protein 14-3-3 testing was ordered and was positive. Clinically, he experienced worsening neurological function after having been COVID-19-positive on admission. The case fulfilled the probable diagnostic criteria for diagnosing PrD. The patient continued to deteriorate and died due to the rapid progression of his condition.

Conclusions:

Our case demonstrates the potential correlation of COVID with neurodegenerative conditions, especially prion disorders. While such cases are highly likely to be due to COVID-19, there is no definite evidence beyond coincidental findings. Future studies might be required to establish this correlation.

Background

PrD are transmissible spongiform encephalopathies manifesting after misfolding prion proteins accumulate within brain cells, resulting in ballooned neurons. Variable types of PrD exist, including Creutzfeldt-Jacob, kuru, iatrogenic, and genetic diseases such as fatal familial insomnia and genetic Creutzfeldt-Jacob and Gerstmann-Sträussler Scheinker syndrome [1,2]. Regardless of the etiology, clinical findings typically involve myoclonus, parkinsonian features, and rapidly progressive dementia. It can be challenging to diagnose PrD due to lack of specific tests. Therefore, diagnostic criteria have been developed to facilitate clinical diagnosis for clinicians [3].

Our literature search found a few cases of PrD after COVID-19 [4–7]. Remarkably, various neurological complications have been recognized in people after contracting COVID-19. While anosmia is considered a regular consequence of COVID, it is typically underrecognized as cranial nerve I dysfunction, a common neurological complication. Other serious post-COVID-19 complications include encephalopathy, meningitis, encephalitis, peripheral neuropathies, and increased risk of cerebrovascular insults and cranial neuropathies, which suggest a neuroinflammatory component of the viral illness [8]. Our case report is unique by highlighting the potential contribution of COVID-19 to PrD.

Case Report

A 62-year-old man developed diffuse bradykinesia, drooling, and dementia 2 months prior to presenting to our hospital. He was noted to have difficulty walking. He was still highly functioning in terms of work until he was found at home on the floor after a fall, unable to walk and confabulating, with intermittent brief rapid involuntary jerks involving 1 limb at a time, suggestive of myoclonic jerks. On admission, he was found to be COVID-19 positive, but was afebrile and asymptomatic aside from the typical COVID respiratory manifestations. He was also diagnosed with rhabdomyolysis based on a CPK value of 3542. Levels of magnesium, ethanol, troponin, acetaminophen, salicylate, ammonia, mushroom Rf212, fentanyl, and 10 panel drug screen panel were normal negative. On the second day of hospitalization, he underwent thyroid function testing (normal), hepatic function testing (mild AST elevations), and initial lumbar puncture, which was remarkable only for protein elevated to 61. Cerebral spinal fluid (CSF) from this lumbar puncture was negative for Escherichia coli K1, Haemophilus influenzae, Listeria monocytogenes, Neisseria meningitidis, Streptococcus agalactiae, Streptococcus pneumoniae, Cytomegalovirus (CMV), Cryptococcus neoformans gattii, Enterovirus, Herpes simplex virus 1 (HSV-1), Herpes simplex virus 2 (HSV-2), Human herpesvirus 6 (HHV-6), Human parechovirus, and Varicella zoster virus (VZV). Testing for HIV, syphilis, B12, TSH, NDMA AB IgG, Glutamic Acid Decarboxylase, and Lyme antibodies were all negative. He had 2 indeterminate QuantiFERON tests, with no findings suggestive of active tuberculosis on chest X-ray. He also underwent CT brain and MRI brain, both of which were repeated twice and were normal without any diffusion restriction. EEG was repeated twice and revealed findings of mild encephalopathy with no sharp-wave complexes. On week 2 of hospitalization, he underwent a second lumbar puncture, which was sent for autoimmune encephalitis panel, TAU protein quantification, 14-3-3 protein levels, and paraneoplastic markers. Of these, 14-3-3 protein was found to be elevated to 3902 (ref. range <30–1999 Au/mL). A diagnosis of probable PrD was established based on the positive result of 14-3-3 protein detection in CSF along with a clinical picture of rapidly progressive dementia with bradykinesia and myoclonus.

Approximately 3 weeks into hospitalization, the patient became progressively mute and had difficulty swallowing soft foods, requiring PEG tube placement. He subsequently became spastic with severe pain on passive flexion extension. Six weeks after admission, the patient was declared dead (Table 1).

Table 1.

Presenting symptoms in our patient and progression of the disease.

Presenting symptoms	1 month into disease course	2 months into disease course	3 months into disease course	4 months into disease course
– Forgetfulness – Confusion – Patient referred for neurological evaluation, did not attend	– Bradykinesia – Frequent drooling – Patient noted manually lifting his legs to walk	– COVID-19 positive – Hallucinations – Dysarthria – Hospitalization	– Diffuse muscle rigidity – Myoclonic jerking – Loss of swallow reflex	– Akinetic mutism – Severe paratonia of all limbs – Reemergence of primitive reflexes – Death

Discussion

We were able to identify 4 cases in the literature (Table 2) comparable to our case with prion disorder happening after COVID-19 infection [4–7]. While 2 cases had neurologic manifestations at onset [4,7], the 2 other cases had CJD-related symptoms at 2 weeks [6] or 2 months [5] after upper-respiratory symptoms. Indeed, 2 cases had COVID-19-related manifestations, with 1 manifesting as fever [6] and the other manifesting as fatigue, hyposmia, and hypogeusia [5]. Counter-intuitively, 2 cases did not have any COVID-19-related manifestations prior to neurologic symptoms [4,7]. However, they tested positive on COVID-19 PCR testing. Most of the cases had CJD-related diagnostic features, including clinical evidence such as rapidly progressing dementia and myoclonus, radiologic features such as cortical ribboning, neurophysiological signs such as periodic lateralized discharges on EEG, and laboratory findings with protein positive 14-3-3 and RT-QuIC, but not all the cases fulfilled most of the criteria.

Table 2.

Post-COVID PrD case reports in the literature.

Case	Age	COVID related manifestations	Clinical manifestations related to CJD	Time to onset of CJD	Tests supportive of CJD diagnosis	Progression	Death
Young 2020 [6]	60	Fever, PCR positive	Confusion, dementia, slowness, paucity of speech with paraphasic errors, unsteady gait with intermittent right-hand clenching, perseveration, anomia, impaired comprehension, and intermittent myoclonic jerks of the right arm	Confusion at onset with worsening over the next 2 weeks	CSF RT-QuIC, 14-3-3 and T-tau Brain MRI diffusion restriction in the left hemisphere and left caudate and thalamus EEG 1 Hz periodic lateralized discharges	Mutism, right hemiplegia, somnolence agitation	2 months later
Beretta 2021 [4]	64	No fever or respiratory symptoms	Confusion, disorientation, moderate aphasia, mild right hemiparesis, and irregular myoclonic jerks at the right limbs, with a Glasgow Coma Scale (GCS) 12 (eyes opening to verbal command, confused, localizing pain, not obeying commands)	Symptoms at onset	14-3-3 positive RT-QuIC negative EEG with left sided lateralized periodic discharges MRI cortical ribbon of the left perisylvian regions	Total recovery	NA
Bernardini 2022 [5]	40	March 2021 persistent fatigue and transient hyposmia and hypogeusia SARS-CoV-2 serology showed positive anti-Receptor Binding Domain (RBD) Spike 1 total antibodies at high titer	Black shadows when closing his eyes, followed by dizziness, difficulty reading and worsening of balance. In June 2021 he noticed loss of coordination of the left arm	2 months	EEG recordings showed diffuse theta slowing with generalized triphasic 1–2 Hz periodic sharp-wave complexes very high NfL levels. high total Tau levels, slightly reduced beta-amyloid 1–42 and normal phospho-Tau-181 and beta-amyloid 42/40 ratio. Real-Time Quaking-Induced Conversion (RT-QuIC) CSF testing for PrP was positive	bedridden	Was alive at the time of publication
Olivo 2022 [7]		COVID swab positive	inability to sign, compromised coordination and paresis to the right leg speech impairments, dystonia and myoclonus. mild right hemiparesis, non-fluent speech and bilateral myoclonus augmented by startle	Neurologic manifestation at onset	MRI scan and highlighted the presence of a diffuse cortical ribboning in DWI periodic triphasic complexes were observed at EEG RT-QuIC results confirmed the probable diagnosis of CJD	Akinetic mutism	Death

Our patient had a clinical picture that fit with PrD, including a rapidly progressive course along with generalized full-body myoclonus. Despite not fulfilling the radiologic and neurophysiological criteria, our patient had a positive 14-3-3 protein in the CSF. As such, he fulfilled the probable diagnostic criteria for CJD, with rapidly progressive dementia along with bradykinesia and myoclonus associated with a positive protein 14-3-3, which fits with the probable diagnostic criteria [3].

The diagnosis of prion disorders is challenging by itself, as definitive diagnosis relies on clinical neurodegeneration along with a histopathological evidence of disease activity within brain parenchyma. While brain biopsy is theoretically the method for a definitive diagnosis, it is invasive and associated with adverse effects, including hemorrhage. The other potential drawback is false-negative results in light of sampling errors as proven by a meta-analysis of brain biopsies with an average diagnostic success rate of 54% [9].

It can be difficult to establish a correlation between PrD and COVID-19 based on laboratory workup. However, this was clinically evident through the timeline of event development, since symptom onset was associated with COVID-19-positivity. It remains difficult to establish a clear correlation because the patient was admitted after a fall but had been developing undefined symptoms reported by coworkers and family members. Whether COVID-19 contributed to his PrD or resulted in the clinical correlation cannot be determined. It is worth noting that in most instances patients with COVID-19 acquire a hyperinflammatory state typically known as cytokine storm, in which excessive inflammatory markers are released within the area of infection. Whether cytokine storm contributes to development of prion disease is yet to be determined. However, excessive protein secretions might also contribute to protein misfolding [10]. SARS-CoV-2 cell entry is facilitated by a viral glycoprotein termed S glycoprotein or S1 spike proteins [11], which plays a role in viral recognition by ACE2R cell membrane receptors [12]. S1 spike proteins were hypothesized to possess prion-like properties with potential contribution to neurodegenerative conditions [13]. This could be the culprit protein playing a role in prion formation. COVID-19 by itself has been described to result in neurodegeneration. However, the exact correlation was not established despite a few reports of Parkinson’s disease [14]. There are definite hypotheses that variable pathogen related-illnesses can result in neurodegenerative conditions, most prominently the Braak hypothesis, wherein gut microbiota provoke protein misfolding and alpha synuclein accumulation, traveling through the vagal nerve to the CNS [15]. It remains difficult to establish such theoretical hypotheses, and the correlation between COVID-19, and in our case it remains hypothetical. While our patient was found to be positive for COVID-19 later in the disease course, our case is important as it suggests a potential correlation between PrD and COVID-19, but this is unproven. The Spanish Flu was followed by an epidemic of Von Economo’s encephalitis [16], and early recognition of potential neurodegenerative conditions after COVID-19 might provide clinical evidence of an association between COVID-19 and these neurologic diseases.

Conclusions

Evidence in the literature about an association between COVID-19 and neurodegeneration is unclear. For the most part, at least clinically, cases were confirmed from the standpoint of acuity to have been the result of COVID-19, but there is no definite biochemical evidence of such a correlation. Nonetheless, it is evident that multiple neurodegenerative conditions might be a result of pathogenic illnesses, most commonly prion disorders. Despite an unclear correlation, it is important that sequala of COVID-19 infection be examined with caution.