|
Salmonella strains
|
|
|
| 14028s WT |
Salmonella enterica serovar Typhimurium strain 14028s |
Lab stock |
| 14028s.ssrB.H12Q
|
14028s ssrB:ssrB.H12Q-catR
|
This work |
| DW637 |
14028s ΔssrB attP::PsifA-lacZ (KanR) |
Desai et al., 2016
|
|
E. coli strains
|
|
|
| TOP10 |
E. coli DH10B |
Saunders lab, MBI |
|
|
|
|
Plasmids
|
|
|
| pMPMA5Ω |
(AmpR) |
Lab stock |
| pMPM_SsrBWT |
pMPMA5Ω plasmid cloned with 6xhis-ssrB (AmpR) |
Feng et al., 2004
|
| pMPM_SsrBc |
pMPMA5Ω plasmid cloned with 6xhis-ssrBc (AmpR) |
Feng et al., 2004
|
| pMPM_SsrB_H28A |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H28A (AmpR) |
This work |
| pMPM_SsrB_H34A |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H34A (AmpR) |
This work |
| pMPM_SsrB_H72A |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H72A (AmpR) |
This work |
| pMPM_SsrB_2H1 |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H28A.H34A (AmpR) |
This work |
| pMPM_SsrB_2H2 |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H28A.H72A (AmpR) |
This work |
| pMPM_SsrB_2H3 |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H34A.H72A (AmpR) |
This work |
| pMPM_SsrB_3H |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H28A.H34A.H72A (AmpR) |
This work |
| pMPM_SsrB_H12A |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12A(AmpR) |
This work |
| pMPM_SsrB_H12Q |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12Q (AmpR) |
This work |
| pMPM_SsrB_H12N |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12N (AmpR) |
This work |
| pMPM_SsrB_H12Y |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12Y (AmpR) |
This work |
| pMPM_SsrB_H12K |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12K (AmpR) |
This work |
| pMPM_SsrB_H12F |
pMPMA5Ω plasmid cloned with 6xhis-ssrB.H12F (AmpR) |
This work |
| pKDS121 |
pKD3 plasmid with ssrB.H12Q inserted upstream the catR gene |
This work |
|
Cell lines
|
|
|
| HeLa |
Human Cervical Adenocarcinoma cell line |
ATCC |
| RAW264.7 |
Murine Macrophage cell line |
Bruno lab, UTMB |
