| SsrB_His28A_FP |
SsrB: substituting His28 to A |
TTACCCTGGCCTGCCTTTAAAATTGTA
|
| SsrB_His28A_RP |
SsrB: substituting His28 to A |
TACAATTTTAAAGGCAGGCCAGGGTAA
|
| SsrB_His34A_FP |
SsrB: substituting His34 to A |
TTTAAAATTGTAGAGGCGGTTAAAAATGGTCTT
|
| SsrB_His34A_RP |
SsrB: substituting His34 to A |
AAGACCATTTTTAACCGCCTCTACAATTTTAAA
|
| SsrB_His72A_FP |
SsrB: substituting His72 to A |
ATTCCTCAATTAGCACAGCGTTGGCC
|
| SsrB_His72A_RP |
SsrB: substituting His72 to A |
GGCCAACGCTGTGCTAATTGAGGAAT
|
| SsrB_H12N_FP |
SsrB: substituting His12 to N |
TTAGTAGACGATAATGAAATCATCA
|
| SsrB_H12N_RP |
SsrB: substituting His12 to N |
GATGATTTCATTATCGTCTACTAATAA
|
| SsrB_H12K_FP |
SsrB: substituting His12 to K |
TTAGTAGACGATAAGGAAATCATCA
|
| SsrB_H12K_RP |
SsrB: substituting His12 to K |
GATGATTTCCTTATCGTCTACTAATAA
|
| SsrB_H12Y_FP |
SsrB: substituting His12 to Y |
TTAGTAGACGATTATGAAATCATCA
|
| SsrB_H12A_FP |
SsrB: substituting His12 to A |
TTAGTAGACGATGCGGAAATCATCA
|
| SsrB_H12Q_FP |
SsrB: substituting His12 to Q |
TTAGTAGACGATCAGGAAATCATCA
|
| SsrB_H12F_FP |
SsrB: substituting His12 to F |
TTAGTAGACGATTTTGAAATCATCATT
|
| SsrB_RP |
SsrB reverse primer |
TTAATACTCTATTAACCTCATTCTTCG
|
| pKDSsrB_FP |
To amplify ssrB.H12Q with homology to pKD3 |
ATATGAATATCCTCCTATGAAAGAATATAAGATCTTATTA
|
| H12Q_CmR_RP1 |
To amplify ssrB.H12Q with homology upstream of catR
|
GGACCATGGCTAATTCCCATTTAATACTCTATTAA
|
| GA_CmR_FP |
To amplify pKD3 with homology to ssrB.H12Q
|
TTAATAGAGTATTAAATGGGAATTAGCCATGGTCC
|
| pKD3_FP |
To amplify pKD3 with homology to ssrB.H12Q
|
GATCTTATATTCTTTCATACTAAGGAGGATATTCATAT
|
| Lam_FP |
To amplify ssrB.H12Q.catR with 40 bp homology upstream of ssrB in 14028s |
ATTACTTAATATTATCTTAATTTTCGCGAGGGCAGCAAAATGAAAGAATA
|
| Lam_RP2 |
To amplify ssrB.H12Q.catR with 40 bp homology downstream of ssrB in 14028s |
CAAAATATGACCAATGCTTAATACCATCGGACGCCCCTGGGTGTAGGCTGGAG
|
| Ex_SsrB_F |
To confirm insertion of ssrB.H12Q.catR in 14028s |
TTGGTATGCTATGTCATAGACA
|
| Ex_SsrB_R |
To confirm insertion of ssrB.H12Q.catR in 14028s |
GTGCGGCATACCAGGGCATC
|
| pMPM_FP |
For sequence check of constructs in pMPMA5Ω |
TACCTGACGCTTTTTATCGC
|
| pMPM_RP |
For sequence check of constructs in pMPMA5Ω |
CTTCTCTCATCCGCCAAAAC
|
|
sseI hairpin |
Used for single-molecule unzipping assay |
5′-Biotin-TTTTTTTTTTTCTATGCGCCAGTCCTTAATGGCATTATCTGAATCGTTAAGTAATTTCTTGTG TGAAATTACTTAACGATTCAGATAATGCCATTAAGGACTGGCGCATAGAGCAGCGTCCCGGGCGGCC
|
