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. 2023 Jul 28;12:e84710. doi: 10.7554/eLife.84710

Figure 5. Severe loss of COX4 associated with increased expression of glucose intracellular transporter in Parl-/- spermatocytes.

Figure 5.

(A) Quantitative immunofluorescence shows decreased expression of COX4 in SCP-1-positive spermatocytes from 5-week-old Parl-/- mice compared to WT littermates (n = 3 for each genotype, 500–1000 SCP-1-positive spermatocytes for each mouse, two-sided Student’s t-test: p=0.0027). Scale bars, 100 µm. Bar graphs indicate average ± SD. (B) Normalized quantification of TOMM20 immunofluorescence in SCP-1-positive primary spermatocytes does not reveal significant differences in mitochondrial mass in the two different genotypes (n = 3 mice for each genotype, 500–1000 SCP-1-positive spermatocytes considered for each mouse; p=0.821). Scale bars, 100 µm. Bar graphs indicate average ± SD. Statistical significance calculated by two-sided Student’s t-test. (C) GLUT1 immunohistochemistry of testis from 5-week-old mice shows prominent overexpression of GLUT1 in arrested Parl-/- spermatocytes, and low levels in WT (arrowheads) (n = 3 for each genotype). Scale bars, 50 µm.