Fig. 2.

Iron uptake into T. gondii-infected mouse eyes (A) Comparison of Pru and RH strain of T. gondii-infected mouse eyes showed similar toxoplasma retinochoroiditis at 7days POI. (B) T. gondii-infected mouse eyes showed toxoplasmic retinochoroiditis (blue arrow heads) and Fe(II) accumulation as detected by FeRhoNox-1 (red) in PRL at 7 and 28 days POI (yellow arrow heads). (C) LA–ICP–MS detected a colloidal gold-positive (Cone opsin-positive) site and revealed the localization of ⁵⁷Fe in the retina of ⁵⁷Fe IVT mice. Note that ⁵⁷Fe mostly accumulated at locations adjacent to the inner/outer segment of photoreceptor cells. (D) ⁵⁷Fe was administered intravitreally (IVT) simultaneous to T. gondii infection.(E) The ⁵⁶Fe/C ratio in the retina of T. gondii-infected mice was not significantly different from that in noninfected eyes, whereas the ⁵⁷Fe/C ratio in the retina of T. gondii-infected mouse was significantly higher than that in noninfected eyes at 7 days POI (n = 6). (F) ⁵⁷Fe was administered intravenously (i.v.) simultaneous to T. gondii infection. (G) The ⁵⁶Fe/C ratio in the retina of T. gondii-infected mice was not significantly different from that in noninfected eyes, and the ⁵⁷Fe/C ratio in the retina of T. gondii-infected mice was not significantly different from that in noninfected eyes at 7 days POI. Both ⁵⁶Fe/C and ⁵⁷Fe/C ratio in the retina of T. gondii-infected mice was significantly higher than that in noninfected eyes at 28 days POI. (n = 6) Data are presented as mean ± SD. AU: gold, INL: inner nuclear layer, ONL: outer nuclear layer, PR: photoreceptor, RGC: retinal ganglion cell, RPE: retinal pigment epithelium, POI: post infection, N.S.: there was no significant difference, IVT: intravitreal injection, i.v.: intravenous injection, Scale bar = 100 μm in (A). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)