Fig. 2.

Sex difference in mitochondrial iron loading and mtROS production by ferroptosis inducers. (A) Male and female P-Hepa were treated with 100 μM FAC for 4 h, followed by staining for mitochondrial Fe²⁺ with 5 μM Mito-FerroGreen (MFG) and mtROS with 5 μM MitoSOX. (B) P-Hepa were treated with 100 nM RSL3 for 4 h, 2 μM FIN56 or 10 μM iFSP1 for 24 h prior to staining with MFG or MitoSOX. Bars, 50 μm.