Fig. 8.

Ovariectomy induces hyperactivation of ERK protecting hepatocytes against ferroptosis. (A) The expression levels of phospho-ERK1/2 (p-ERK) and phospho-Akt (p-Akt) were determined in P-Hepa of OVX and sham-female mice. (B) OVX hepatocytes were treated for 4 h with 100 μM FAC in the presence or not of 5 μM PD0325901 (PD), and then stained for PI. (C) The expression of p-ERK and p-Akt in male P-Hepa and AML12 cells. (D) AML12 cells were treated for 24 h with or without FAC or PD, followed by PI staining. The percentages of PI⁺ cells (B and D) were quantified and are shown as mean ± SEM (n = 9 fields of 3 independent experiments). (E) AML12 cells were treated for 4 h with or without PD, and the mRNA expression of FSP1 was determined by qRT-PCR. Data are mean ± SEM (n = 3). *, P < 0.01. Bars, 200 μm.