Fig. 8. Co-recruitment of Mediator complex and human CTD into condensates.

a Differential interference contrast and fluorescence microscopy of the phase separation of hCTD and the human Mediator complex. Different mixtures and conditions are indicated with vertical bars. hCTD was labeled with Alexa Flour 488 (AF488; green), and the human mediator complex hMED with Alexa Flour 647 (red). b–e Structure of the mediator-bound preinitiation complex (PDB id 7ENC²⁵). Some proteins were omitted for better visualization in panel (b). The structure of the hMED-bound CTD is displayed in (b, inset). Panels c–e highlight Tyr-Tyr (c), Pro-Pro (d), and Tyr-Pro (c and e) contacts between mediator subunits and hCTD. Micrographs are representative of 3 independent biological replicates. Scale bar, 5 µm.