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. 2023 Sep 25;14:5534. doi: 10.1038/s41467-023-41095-y

Fig. 6. BM-derived THBS1-expressing cells are recruited by CXCL12 signaling.

Fig. 6

a qRT-PCR analyses in BM cells (n = 3 mice). b FACS analyses in the BM and peripheral blood cells (n = 3 mice). Positive cell numbers in 10000 total cells are shown. c qRT-PCR analyses of sorted cells from BM or peripheral blood of MTO-bearing mice (n = 3 mice). d, e qRT-PCR analyses of sorted cells from MTO tumors in GFP-BM mice (n = 3 mice). f Co-immunostaining of the MTO tumors in GFP-BM mice (n = 3 mice). g qRT-PCR analyses of the MTO tumors in WT-BM WT (n = 5), Thbs1-/--BM Thbs1-/- (n = 6), and WT-BM Thbs1-/- chimeric mice (n = 5). h Macroscopic numbers of metastasis (n: WT-BM WT = 13, Thbs1-/--BM Thbs1-/- = 5, WT-BM Thbs1-/- chimeric mice = 15). i Bioluminescence imaging (top) and H&E staining of liver (bottom) in indicated mice with MTO implantation. j Immunostaining in MTO tumors and positive cell proportion (n = 5 mice). k qRT-PCR analyses in BM cells of MC38- or MTO-bearing mice (n = 3 mice). l Heatmap in TCGA (n: CMS1 = 85, CNS2 = 132, CMS3 = 78, CMS4 = 184). m qRT-PCR analyses in indicated orthotopic tumors in WT mice (n = 3 mice). n Transcript levels in TCGA. o, qRT-PCR analyses of sorted cells from BM of MTO-bearing mice (n = 3). p, FACS analyses of proportions among THBS1+ cells in MTO-bearing WT mice (n = 3). q, Co-immunostaining in WT tumors (n = 3 mice). r–u CXCL12 inhibitor (LIT-927) treatment, following orthotopic MTO implantation (n: control = 8, LIT-927 = 7). Schematic representation (r), qRT-PCR analyses (s, n = 4), bioluminescence imaging t, and quantification of macroscopic numbers of metastases u. Arrows in i, t: primary lesions (white), distant metastases (yellow). Dash lines denote liver metastases in i. Scale bars, 10 μm f, j, q, 500 μm i. Mean ± SEM. Two tailed, Mann–Whitney test n, one-way ANOVA p, or tow tailed, unpaired Student’s t test (the rest). Source data are provided as a Source Data file.