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. 2023 Sep 25;13:16058. doi: 10.1038/s41598-023-43354-w

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© The Author(s) 2023, corrected publication 2023, 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Visualization of C. acnes EVs by different microscopy techniques. (A) Transmission Electron Microscopy of C. acnes SLST H1 EVs and A1 EVs (bar 500 nm). (B) Nanoparticle Tracking Analysis of C. acnes SLST A1, H1 and H2 EVs. Prior to the measurement, the samples were diluted in PBS buffer at 1:5000, 1:1000 and 1:1000 respectively. EVs have a mean diameter of 96.8 ± 0.5 nm, 100.7 ± 0.7 nm and 133.0 ± 2.3 nm respectively. EV-concentration is expressed as a number of particles per mL on the y axis. All data represent the mean of three independent experiments ± standard error.