Table 3.
ANOVA results showing the significance of factors and factorial interactions on DNA extraction concentration. Amount of proteinase K did not significantly impact DNA yield, subsequently its interactions with other factors are not shown here. DFn: the degree of freedom for the numerator of the F ratio, DFd: the degree of freedom for the denominator, ***P < 0.001 and * P < 0.5
| Factors | DFn | DFd | F | p |
|---|---|---|---|---|
| time of digestion (TOD) | 1 | 288 | 61.206 | 9.82E-14*** |
| amount of ATL buffer (AAB) | 1 | 288 | 105.539 | 2.68E-21*** |
| time of lysis (TOL) | 2 | 288 | 28.257 | 6.23E-12*** |
| amount of proteinase K | 2 | 288 | 0.363 | 0.696 |
| TOD: AAB | 1 | 288 | 0.226 | 0.635 |
| TOD: TOL | 2 | 288 | 3.95 | 0.02* |
| AAB: TOL | 2 | 288 | 1.142 | 0.321 |
| TOD: AAB: TOL | 2 | 288 | 1.292 | 0.276 |