Osteogenic effect of each group of scaffolds in vitro. (a) Expression of osteogenesis-related genes at 3 and 7 days; (b) ALP staining of BMSCs cultured with extracts for 7 days; (c) ARS staining of BMSCs cultured with extracts for 14 days; (d) Western blotting of COL-1, RUNX-2, OPN, ALP in BMSCs after culturing with the extract for 3 days; (e) Quantitative analysis of COL-1, RUNX-2, OPN, ALP compared with GAPDH. Data are shown as mean ± standard deviation. **p < 0.01, ***p < 0.001.