Fig. 7.

Effects of different groups of nanofibrous membranes on osteoclasts. (a and b) Changes in the expression levels of osteoclast-related genes TRAP and cathepsin K after induction of RAW264.7 cells using the RANKL; (c and d) expression levels of TRAP and cathepsin K in different groups of RAW264.7 after cultured with membranes for five days; (e) quantitative analysis of the TRAP fluorescence intensity; (f) cytoskeletal staining of RAW264.7 cells and osteoclasts; and (g) immunofluorescence staining for TRAP. Data are presented as mean ± SD (n = 3). *p < 0.05 and ***p < 0.001.