Fig. 6.

The lineage-specific multifunctional scaffold induced zone-specific col 2 and alkaline phosphatase (ALP) expression, as determined by staining of each layer. A. The effect of the lower layer on the expression of ALP and calcium deposition (alizarin red staining) after 14 days of culture determined by transwell assays. B. Schematic illustration of the culture process with the bilayered constructs in the transwell assays. C. Differential expression of chondrogenesis- and osteogenesis-related genes induced in vitro after treatment with the bilayer constructs. D-E. Immunofluorescence staining of cartilage (col 2)- and bone (ALP)-related proteins. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)