Figure 4.

SQLE inhibition destabilizes c‐Myc and impairs its transcriptional activity. a) GSEA analysis shows significant enrichment of genes in the MYC_TARGETS_V1 molecular signature in SCC‐23^cisR cells without SQLE depletion. b) Heatmap depicting aberrant expression of c‐Myc target genes upon SQLE depletion in SCC‐23^cisR cells. c) qRT‐PCR analysis of representative c‐Myc‐induced target genes in SCC‐1^cisR cells following SQLE depletion (n = 4). d) Expression of c‐Myc and SQLE in SCC‐1^cisR and SCC‐23^cisR cells upon SQLE knockdown. e,f) SQLE‐depleted SCC‐1^cisR and SCC‐23^cisR cells treated with MG132 (20 µm, 8 h) before harvest, with SQLE and c‐Myc expression analyzed by western blotting. g,h) Time‐course analysis of c‐Myc and SQLE expression in SCC‐1^cisR and SCC‐23^cisR cells with or without SQLE depletion. Cells treated with CHX (100 µg mL⁻¹) for varying periods. i,j) SCC‐1^cisR or SCC‐23^cisR cells with or without SQLE overexpression were treated with 100 µg mL⁻¹ CHX for indicated time periods; western blotting performed to analyze dynamic changes in c‐Myc and SQLE. Data were presented as mean ± SD. ns (not significant), **p < 0.01, ***p < 0.001 for Student's t‐test.