Skip to main content
. 2023 Jul 25;10(27):2206878. doi: 10.1002/advs.202206878

Figure 6.

Figure 6

SQLE depletion reduces cholesterol content in lipid rafts of HNSCC cells and suppresses phosphorylation of raft‐associated Akt. a,b) Relative cholesterol content in lipid rafts from cells with indicated treatments (n = 3). c,d) Time‐course analysis of p‐Akt S473 and Akt expression in lipid raft and nonraft components of SCC‐1cisR and SCC‐23cisR cells treated with DMSO or NB‐598 (1 µm). Western blot analyses performed with indicated antibodies. β‐actin or Flot2 serves as internal control for determining protein expression in nonraft components or lipid rafts, respectively. e,f) Expression of p‐Akt S473 and Akt in lipid raft and nonraft components of SQLE‐depleted cells or CTRL cells with or without addition of exogenous cholesterol. Data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 for Student's t‐test and one‐way ANOVA test.