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. 2023 Sep 6;26(10):107830. doi: 10.1016/j.isci.2023.107830

Figure 3.

Figure 3

Pathological changes and viral load in the lungs

(A and B) 20× magnification of hematoxylin and eosin staining of lung sections from two macaques that survived H5N1 challenge, (A) macaque 147-19, which survived with only mild disease, (B) macaque 154-19 which had severe respiratory disease but survived. Scale bar is 100 μm.

(C) Graph in (C) shows percent alveolar space (y axis) with average temperature elevation (x axis); survivors (S) are plotted as solid circles; non-survivors (NS) are open circles. Lines show the linear regression analysis of survivors with the 95% confidence intervals.

(D and E) viral load in the lung at necropsy; macaques that survived are plotted as solid circles; non-survivors are open circles. UL = upper right lung; ML = middle right lung; LL = lower right lung. (D) infectious virus as measured by plaque assay of lung tissues; (E) viral RNA as measured by qRT-PCR of lung tissues.

(F) Expression of viral nucleoprotein (green) with that of cytokeratin (red) in the lung of 154-19, a macaque that survived severe H5N1 infection (400× magnification).