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. 2023 Sep 6;26(10):107830. doi: 10.1016/j.isci.2023.107830
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

anti-influenza A nucleoprotein BEI Resources Clone DPJY03
Mouse anti-pan cytokeratin Abcam clone AE1/AE3
mouse anti-human CD163 ThermoFisher/eBioscience eBioGHI/61
goat anti-mouse IgG2a Alexa Fluor 647 ThermoFisher A-21241
goat anti-mouse Alexa Fluor 546 ThermoFisher A-11030

Bacterial and virus strains

H5N1 (A/Vietnam/1203/2004) Daniel Perez, University of Georgia N/A

Biological samples

Cynomologus macaque lung & bronchoalveolar lavage This paper N/A
Cynomolgus macaque plasma This paper N/A

Chemicals, peptides, and recombinant proteins

TRIzol Invitrogen 15-596-018

Critical commercial assays

Anti-NP inhibition assay Virusys IAV207-2
RNA Clean and Concentrator Zymo R1018
Reliance One-Step Multiplex Supermix BioRad 12010220

rowheadDeposited data

Raw telemetry & plethysmography data Available on request N/A
Exported & analyzed telemetry & plethysmography data Available on request N/A
ELISA, qRT-PCR, PRNT, plaque assay data Available on request N/A
Aerosol exposure data Available on request N/A
Immunohistochemistry images & alveolar space analysis data Available on request N/A

rowheadExperimental models: Cell lines

MDCK cells ATCC CCL-34
Experimental models: Organisms/strains
Cynomolgus macaques, Mauritius origin VRC N/A

Oligonucleotides

Influenza A primers and probes BEI Resources NR-15592

Software and algorithms

Prism 9 GraphPad https://www.graphpad.com/
Ponemah 6.5 Data Sciences International http://datasci.com
Finepointe 2.8 Data Sciences International http://datasci.com
MatLab 2020a MatLab https://www.mathworks.com/products/matlab.html
NIS-ElementsAR v5.30.01 Nikon https://www.microscope.healthcare.nikon.com/products/software/nis-elements
ImageJ NIH https://imagej.nih.gov/ij/

rowheadOther

Temperature data analysis code Reed Lab https://github.com/ReedLabatPitt/Reed-Lab-Code-Library
Quadrivalent influenza vaccine + Addavax Quadrivalent influenza vaccine: Seqirus. Addvax: Invivogen Quadrivalent influenza vaccine: Afluria 2018-19. Addavax: vac-adx-10