Antibodies |
|
anti-influenza A nucleoprotein |
BEI Resources |
Clone DPJY03 |
Mouse anti-pan cytokeratin |
Abcam |
clone AE1/AE3 |
mouse anti-human CD163 |
ThermoFisher/eBioscience |
eBioGHI/61 |
goat anti-mouse IgG2a Alexa Fluor 647 |
ThermoFisher |
A-21241 |
goat anti-mouse Alexa Fluor 546 |
ThermoFisher |
A-11030 |
|
Bacterial and virus strains |
|
H5N1 (A/Vietnam/1203/2004) |
Daniel Perez, University of Georgia |
N/A |
|
Biological samples |
|
Cynomologus macaque lung & bronchoalveolar lavage |
This paper |
N/A |
Cynomolgus macaque plasma |
This paper |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
TRIzol |
Invitrogen |
15-596-018 |
|
Critical commercial assays |
|
Anti-NP inhibition assay |
Virusys |
IAV207-2 |
RNA Clean and Concentrator |
Zymo |
R1018 |
Reliance One-Step Multiplex Supermix |
BioRad |
12010220 |
|
rowheadDeposited data
|
|
Raw telemetry & plethysmography data |
Available on request |
N/A |
Exported & analyzed telemetry & plethysmography data |
Available on request |
N/A |
ELISA, qRT-PCR, PRNT, plaque assay data |
Available on request |
N/A |
Aerosol exposure data |
Available on request |
N/A |
Immunohistochemistry images & alveolar space analysis data |
Available on request |
N/A |
|
rowheadExperimental models: Cell lines
|
|
MDCK cells |
ATCC |
CCL-34 |
Experimental models: Organisms/strains |
Cynomolgus macaques, Mauritius origin |
VRC |
N/A |
|
Oligonucleotides |
|
Influenza A primers and probes |
BEI Resources |
NR-15592 |
|
Software and algorithms |
|
Prism 9 |
GraphPad |
https://www.graphpad.com/ |
Ponemah 6.5 |
Data Sciences International |
http://datasci.com |
Finepointe 2.8 |
Data Sciences International |
http://datasci.com |
MatLab 2020a |
MatLab |
https://www.mathworks.com/products/matlab.html |
NIS-ElementsAR v5.30.01 |
Nikon |
https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
ImageJ |
NIH |
https://imagej.nih.gov/ij/ |
|
rowheadOther
|
|
Temperature data analysis code |
Reed Lab |
https://github.com/ReedLabatPitt/Reed-Lab-Code-Library |
Quadrivalent influenza vaccine + Addavax |
Quadrivalent influenza vaccine: Seqirus. Addvax: Invivogen |
Quadrivalent influenza vaccine: Afluria 2018-19. Addavax: vac-adx-10 |