Table 1.
Steady state kinetics data for PAT1 enzymes with varied anthranilate
| PAT1 protein | KM (μM) | kcat (min−1) | kcat/KM (M−1s−1) | Ki (mM) |
|---|---|---|---|---|
| Arabidopsis thaliana | 20.6 ± 2.0 | 7.9 ± 0.1 | 6360 | 4.3 ± 0.4 |
| Citrus sinensis | 22.0 ± 3.3 | 7.0 ± 0.2 | 5320 | 4.1 ± 0.6 |
| Juglans regia | 38.7 ± 3.5 | 3.3 ± 0.1 | 1420 | >5 |
| Physcomitrium patens | 31.8 ± 4.4 | 3.4 ± 0.1 | 1790 | >5 |
| Pistacia vera | 32.1 ± 4.7 | 2.4 ± 0.1 | 1240 | 1.8 ± 0.4 |
| Selaginella moellendorffii | 189 ± 35 | 5.1 ± 0.3 | 450 | 3.6 ± 0.8 |
KM apparent, kcat, and kcat/KM values reported for anthranilate concentrations between 0 and 0.75 mM. Values shown are the mean ± standard error for n = 3.