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. 2023 Sep 13;26(10):107897. doi: 10.1016/j.isci.2023.107897

Figure 3.

Figure 3

CircSOBP inhibits glycolysis and mitochondrial respiration in glioma cells

(A) Heatmap of pathway enrichment analysis of differential metabolites in circSOBP overexpression group (pEGFP-C1-circSOBP) and control group (pEGFP-C1) of U87 cells.

(B) Concentrations of pyruvate and lactate between circSOBP overexpression group and control cells.

(C) Concentrations of pyruvate and lactate between circSOBP-silenced and control cells.

(D) Oxygen consumption rate (OCR) indicators, including basal respiration and ATP production levels, were measured in U87 and U251 cells after overexpression of circSOBP.

(E) Overexpression of circSOBP in U87 and U251 cells and detection of extracellular acidification rate (ECAR) as an indicator of inferred glycolytic flux and glycolytic capacity.

(F) Oxygen consumption rate (OCR) was used as an indicator of oxidative phosphorylation (OXPHOS) to detect basal respiration and ATP production levels after silencing of circSOBP in U87 and U251 cells.

(G) Extracellular acidification rate (ECAR) was used as an index to infer glycolytic flux and glycolytic capacity to detect knockdown of circSOBP in U87 and U251 cells. All statistics of error bars, S.E.M. from three independent experiments. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by two-tailed Student’s t test.