Figure 7.

CircSOBP inhibits tumorigenesis and glycolysis as well as promotes the production of IFNs in vivo

(A) Illustration of the tumor transplantation timelines when treated with circSOBP.

(B) Expression level of circSOBP in glioma cells after transduction with overexpression lentivirus.

(C) Bioluminescence imaging of intracranial xenograft tumors in BALB/c nude mice is shown. Images were obtained after 5 min by intraperitoneal injection of 100 μL (25 mg/mL) of D-Luciferin. Seven days later, the same experimental procedure was performed to obtain images again.

(D) Kaplan-Meier plots of overall survival of mice with high circSOBP levels and controls.

(E) HE staining was used to detect the relative area of tumors in cross-sections of mouse brains.

(F) Quantitative analysis of the mean fluorescence intensity (left) and growth rate (right) of brain tumor areas in circSOBP overexpression and control groups of BALB/c nude mice.

(G) The size (left) and growth rate (right) of brain tumor areas (ROI Pixels) in circSOBP overexpression and control BALB/c nude mice were measured.

(H) Bioluminescence imaging of the C57/BL6J glioma model mouse with adeno-associated viral fluid alone or co-injected with Poly(I:C).

(I) Fluorescence intensity and area were used as indicators for the quantitative analysis of tumor size.

(J) Kaplan-Meier plots show overall survival of mice treated with circSOBP/Poly(I:C) together or alone.

(K) IFNs concentrations in the tumor tissues of the grouped mice indicated.

(L–N) The levels of glycolysis and energy metabolism in the tumor tissues of the grouped mice indicated. Data (L) suggest pyruvate contents. Data (M) imply lactate production. Data (N) are indicative of ATP concentrations. All statistics of error bars, S.E.M. from three independent experiments. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 by two-tailed Student’s t test.