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. 2023 Sep 1;299(10):105209. doi: 10.1016/j.jbc.2023.105209

Figure 1.

Figure 1

AMPK kinases TAK1 and STK11 (LKB1) do not mediate M3 mAChR-induced AMPK phosphorylation.A and B, cells were preincubated with 5z-7-oxozeaenol (100 nM) for 15 min then stimulated with (A) PD 102807 (100 μM; t = 20 min) or (B) MCh (100 μM; t = 5 min). C, following siRNA transfection against STK11 (as described in Experimental procedures), cells were stimulated with PD 102807 or MCh. D, STK11 knockdown was confirmed by immunoblotting. Representative blots are shown. Loading was corrected for β-actin or total AMPK. Data are means ± SD. ∗p < 0.05, ∗∗∗ p < 0.001 versus vehicle stimulation; ##p < 0.01 versus mock-transfected PD 102807; one-way ANOVA followed by Bonferroni multiple comparison test. AMPK, AMP-activated protein kinase; LKB1, liver kinase B1; mAChR, muscarinic acetylcholine receptor; MCh, methacholine.