Reagent	Final concentration	Amount
Tris-HCl pH = 8 (0.5 M)	10 mM	400 μL
NaCl (5 Μ)	150 mM	600 μL
EDTA pH = 8 (0.5 M)	2 mM	80 μL
Triton-X (100%)	1%	200 μL
SDS (10%)	0.1%	200 μL
Nuclease-free water	N/A	18.520 mL
Total	N/A	20 mL