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. 2023 Sep 12;17:1231584. doi: 10.3389/fnins.2023.1231584

Figure 1.

Figure 1

Neuronal and mitochondrial phenotypes in live HSP-SPG7 patient neural progenitors and mature cortical neurons. High throughput imaging and analysis was used to evaluate neuron and mitochondrial morphology. (A) Shows the timeline to generate neural progenitors and mature cortical neurons. (B–G) Images of live control and patient neural progenitors and mature cortical neurons labelled with calcein – to identify viable cells, TMRM – to identify mitochondrial and hoechst – to identify nucleus. (H–M) Images of the cells presented in (B–G) with with TMRM and Hoechst label without the calcein label. (N–S) Neurites were segmented and identified using automated image analysis in control and patient neural progenitors and mature cortical neurons. (T–X) Multiple parameters of neurite morphology were analyzed in control and patient neural progenitors and mature cortical neurons. These parameters include neurite length (T), extremities (U), branching (V), segments (W) and roots (X). (Y–B1) Multiple parameters of mitochondrial morphology and membrane potential were analyzed in control and patient neural progenitors and mature cortical neurons. These parameters include (Y) mitochondrial area, (Z) perimeter, (A1) length, (B1) width and TMRM intensity (C1). (D1,E1) Cell viability (D1) and (E1) neurite degeneration in Day 10 mature control and patientneurons. Data is presented as Mean ± SEM. Scale bar: 100 μm.