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. 2023 Sep 13;86(9):2151–2161. doi: 10.1021/acs.jnatprod.3c00363

Figure 4.

Figure 4

CBD induces apoptosis in noncancerous prostate cells. (A) Noncancerous prostate epithelial cells (PWR-1E, RWPE-1) were treated with CBD (0–100 μM) for 72 h in the absence of serum. Cell viability was measured using the MTT assay. (B) Representative images of PWR-1E cells treated with vehicle or 1 μM CBD for 72 h, stained with Hoechst 33342 (blue), YO-PRO (green), and PI (red). (C) The effect of CBD on PWR-1E cell confluency, nucleus area, and nucleus circularity was assessed using high-content fluorescence microscopy. (D) Fractions of early apoptotic, late apoptotic, and healthy PWR-1E cells were measured by fluorescence microscopy, using YO-PRO and PI staining. Data are represented as mean ± SD calculated from at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the vehicle control.