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. 2023 Jul 26;8:327. [Version 1] doi: 10.12688/wellcomeopenres.19617.1

Figure 4. Polymerase alpha subunits Pol1 and Pol12 are CDK phosphorylated in S-phase.

Figure 4.

A) Scale diagram of the two largest subunits of polymerase alpha from yeast. * refers to CDK consensus sites. B) Western blot of the indicated strains released from G1 phase (0) into S-phase for the indicated times. All 13 serines/threonines within CDK consensus sites are mutated to alanine in the pol1-13A mutant. C) As b), except with the Pol12 tagged strains. All 12 serines/threonines within CDK consensus sites are mutated to alanine in the pol12-12A mutant. D) As b), except cells were released from G1 phase into S-phase with 200 mM HU. E) As c), except cells were released from G1 phase into S-phase with 200 mM HU.