Fig. 3. Comet images of cells isolated from mouse tumors: (A) control and (B–D) treated with unloaded LNCs, free TQ, and TQ-LNCs (at 5 mg kg⁻¹ of TQ), respectively. Cells were stained with ethidium bromide (0.02 mg mL⁻¹) and then observed by epifluorescence microscopy using an FITC filter. Magnification = 200×, scale bar = 100 μm. DNA damage in comet cells contains a tail and a head like a comet; apoptotic cells have a large tail and a small head. (E) Effect on total DNA damage assessed through the alkaline comet assay in tumor cells (a: P < 0.01 versus control group, b: P < 0.05 versus TQ group).