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Butyrate-induced inhibition of C. difficile growth is dependent on the metabolic environment. Growth curves of C. difficile strain 630 [log10(OD600)] over 24 h in minimal media (CDMM) in the presence of a single sugar supplemented with (red) and without (black) 25 mM butyrate (n = 3 per condition). Statistical significance calculated using Dunnett’s test: *P-value <0.05; **P-value <0.01; ***P-value <0.001.
