Fig 2.

Conservation, domain organization, and homology model of S. aureus GtrR. (A) Homology model of a S. aureus GtrR homodimer generated using SWISS-MODEL with GtrR from Arabidopsis thaliana as a template (61, 62). Residues selected for mutagenesis are highlighted. On the left monomer, domains are colored to match the domain organization modeled in B. On the right, amino acids are colored according to the degree of conservation. A scale of variable (teal) to conserved (purple) residues is indicated in the figure. (B) Domain organization (bottom bar: Catalytic, pink; NADPH binding, green; Spinal Helix, light orange; Dimerization, dark orange) and conservation of S. aureus GtrR primary sequence compared to increasing taxonomic levels (top bars, colored as in A).