Fig 7.
Stk1 and Stp1 impact heme stress signaling within S. aureus. (A) Growth as measured by OD600 was monitored over time for S. aureus strains grown in TSB containing either vehicle (i) or 15 µM heme (ii, iii). Prior to the measured growth, the strains were pre-grown to stationary phase in TSB containing vehicle (i, ii) or 2 µM heme (iii). (B) Total activation of luciferase expression from the hrtAB promoter (Phrt) from strains grown in TSB containing 2 mM δ-aminolevulinic acid (ALA), 2 µM heme, or vehicle. Time course data used to determine total promoter activation are graphed in Fig. S4. (A and B) Graphed are the averages of means from three independent experiments performed in biological triplicate ± SEM. Significance was calculated using two-way ANOVA with Tukey’s test for multiple comparisons using the comparisons listed in Table 3.