Fig 8.

Stk1 is important for full heme synthesis in a hemX mutant. Heme was quantified by HPLC in S. aureus WT and mutant strains. Data are representative of the averages of means normalized to the amount of heme measured in WT from two independent experiments performed in biological triplicate ± SEM. “n.d.” indicates that no signal was detected. Significance was calculated in Graphpad Prism 8 using a two-way ANOVA with Welch’s test for multiple comparisons between the strain pairs listed in the table on the right with ***P = 0.001; ****P < 0.0001; ns, non-significant; --, comparison not made. No comparison was made between WT and ΔgtrR since Welch’s test cannot be performed on samples with identical values.