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Specific primers used to determine numbers of individual bacteria and the total number of bacteria.
| Target | Primer name | Primer sequence (5′‐3′) |
|---|---|---|
| Faecalibacterium prausnitzii | Praus‐F480 | CAGCAGCCGCGGTAAA |
| Praus‐R631 | CTACCTCTGCACTACTCAAGAAA | |
| Akkermansia muciniphila | Akk.muc‐F | CAGCACGTGAAGGTGGGGAC |
| Akk.muc‐R | CCTTGCGGTTGGCTTCAGAT | |
| Bifidobacterium spp. | F‐Bifid09c | CGGGTGAGTAATGCGTGACC |
| R‐Bifid06 | TGATAGGACGCGACCCCA | |
| Bacteroides spp. | Bacter11 | CCTWCGATGGATAGGGGTT |
| Bacter08 | CACGCTACTTGGCTGGTTCAG |
