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. 2023 Sep 26;23:215. doi: 10.1186/s12935-023-03076-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Immune related analysis. A The mean proportion of 22 immune cells in the Target database. B Violin diagram of the difference in expression of 22 immune cells infiltration between the high and low expression groups of DIO3OS in 85 osteosarcoma samples in the TARGET set. Blue represents the low expression group, red represents the high expression group. C Correlation heat map of 22 immune cells in the TARGET set. D In low expression of DIO3OS group, CD200 and TNFRSF25 expression were inhibited and CD44 expression were increased. E–G Scatter plots of the expression correlation between CD44, CD200, and TNFRSF25 and DIO3OS. P-value of less than 0.05 is considered statistically significant (ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001)