Figure 4.

Virtual staining enables special stain region-of-interest highlight and stain blending. Virtual staining allows to highlight different regions of a single label-free tissue with multiple stains, simultaneously. (a) For example, the glomerular regions can be instantly stained with Jones’ stain, on top of the virtual H&E staining of the background tissue. This way, label-free tissue samples can be virtually stained with a panel of stains, following a predefined microstructure map that is decided by, e.g., a diagnostician. (b) An example of a ROI stained using H&E, Jones, and a mixture of both H&E and Jones stains (performing stain blending).

(a).

(b).