FIGURE 2.

FLIm provides optical contrast between tumor and white matter. (A) Surgical field-of-view with the FLIm scanned area overlaid with the 470-nm average lifetime for a region of tumor, normal cortex, and normal white matter, and corresponding H&E images (×40 magnification). Tumor tissue is histologically characterized by high cellular density. Cortex tissue is composed of neuron bodies (black arrowheads) and appears darker than white matter. Glial cells are present in the white matter (white arrowheads). (B) Average lifetimes and (C) spectral intensity ratios of tumor (red), cortex (blue) and white matter (green) across the three spectral channels. Each point corresponds to the median of each scan. The mean and standard deviation of each tissue group are indicated by the error bars. *p-value < 0.01; non-significant statistics are left unmarked. N indicates the number of patients; n indicates the number of scans