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. 2023 Sep 11;15(17):9086–9104. doi: 10.18632/aging.205018

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Copyright: © 2023 Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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SH2D4A affects proliferation and migration of glioma cells. (A) Immunohistochemical staining of SH2D4A in glioma (data from HPA). (B, C) qRT-PCR and western blot analysis of SH2D4A knockdown efficiency in U87 MG cells. (D) Analysis of proliferation of control and SH2D4A-deficient U87 MG cells by CCK8 assay. (E, F) Representative images and statistical analysis of EdU assay in control and SH2D4A-deficient U87 MG cells. (G, H) Representative images and statistical analysis of cell migration assay in control and SH2D4A-deficient U87 MG cells at the indicated times. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.