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. 2023 Sep 14;8(10):1834–1845. doi: 10.1038/s41564-023-01469-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, A segmented tomogram of a PT, showing ER (transparent blue), a vesicle (V, transparent magenta) and ribosomes (R, white). The PT wall (PTW) and plasma membrane (PM) are transparent grey. b, A subtomogram average of the S. lophii ribosome dimer (composite of two half-dimers) at 10.8 Å resolution, showing the SSU in shades of pink and the LSU in shades of blue. c, Organization of ribosome dimers inside a PT showing the original tomographic slice on the left and subtomogram averages of ribosome dimers placed back into the tomogram on the right (various colours). d,e, Cross-sections through the PT from areas indicated by the dotted lines (designated d and e) in c.