Fig. 2. PgCoV GD strain displays a host range similar to that of SARS-CoV-2.
a, GFP signals were observed in DBT9 cells expressing ACE2 orthologues or empty vector (E.V.) infected with icPgCoV-GFP at 24 h post infection. Scale bar, 100 μM. b, Western blot analysis of ACE2 orthologue or E.V. expression from 15 mammalian species in DBT9 cells. a and b were repeated twice with the same results. c, In vitro potency of RDV, EIDD and nirmatrelvir against icPgCoV in A549-hACE2 cells based on RLU of icPgCoV replication in the presence of decreasing concentrations of RDV, EIDD and nirmatrelvir in A549-hACE2 cells. Quantitation of icPgCoV-nLuc replication as measured by nLuc in technical triplicates. Shown are the IC50 values of RDV, EIDD and nirmatrelvir, n = 3 replicates per group, data are mean ± s.d.