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. 2023 Sep 26;14(9):635. doi: 10.1038/s41419-023-06161-9

Fig. 6. ELOVL6, SCD1, RIP-1, MLKL, TNF-α, and CASP1 mRNA expression in liver tissue of NAFLD patients.

Fig. 6

mRNA and cellular expression proteins. Data were obtained from RNA sequencing analysis, as downloaded from the Gene Expression Omnibus database (GSE130970), of liver tissue obtained from 72 patients with NAFLD and from 6 controls (CTRL). Mean expression data are expressed as TPM (transcripts per million) (A). Representative western blotting analysis of ELOVL6, SCD1, RIP-1, MLKL, TNF-α, CASP1 proteins, and marker protein GAPDH in Hepa-RG cells treated with NAFLD-Exo and Healthy-Exo samples (B). Semiquantitative evaluation of proteins expression level in the Hepa-RG cell line treated with NAFLD-Exo and Healthy-Exo samples, by video-densitometry analysis of proteins bands on western blotting. GAPDH protein band was used for the normalization of the targeted protein bands for each sample considered. *P < 0.005 and **P < 0.001 vs healthy subjects or untreated cells (CTR) (C).