Figure 1.

HTT is required for axon regeneration

(A) In situ hybridization showing HTT mRNA expression in the adult retina in intact and injured (3dpc) conditions.

(B) Fluorescent in situ hybridization and quantification of HTT mRNA expression in intact and 3dpc RBPMS⁺ RGCs.

(C) Immunofluorescence and quantification of HTT protein expression in intact and 3dpc RBPMS⁺ RGCs.

Data are represented as mean +/− SEM. Mann-Whitney U tests, ^∗∗∗p value < 0.001.

(D) Immunofluorescence and quantification showing HTT protein expression in Pten^fl/fl+AAV2-Cre or AAV2-Plap in intact and 3dpc RBPMS⁺ RGCs.

(E) Whole optic nerve confocal images showing CTB⁺ regenerating axons in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap conditions at 14dpc. The injury site is indicated by a red star.

(F) Quantification of integrated fluorescence intensity along the optic nerve. The top graph gives the multiple comparisons test between Pten^fl/fl+AAV2-Cre and Pten^fl/flHTT^fl/fl+AAV2-Cre conditions.

(G) Whole-mount retina confocal images showing RBPMS⁺ and pS6⁺ RGCs in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap intact conditions.

(H) Quantification of RBPMS⁺ RGCs per mm² retina.

(I) Quantification of pS6⁺ RGCs per mm² retina.

(J) Whole-mount retina confocal images showing RBPMS⁺ and pS6⁺ RGCs in Pten^fl/fl+AAV2-Cre, Pten^fl/flHTT^fl/fl+AAV2-Cre, and Pten^fl/flHTT^fl/fl+AAV2-Plap conditions at 14dpc.

(K) Quantification of RBPMS⁺ RGCs as a percentage of intact condition.

(L) Quantification of pS6⁺ RGCs per mm² retina.

Data are represented as mean +/− SEM. One-way ANOVA with Bonferroni multiple comparisons test; ^∗p value < 0.05, ^∗∗p value < 0.01, and ^∗∗∗p value < 0.001; ns, not significant.