Figure 3.

HTT interacts with ribosomes but does not control global translation

(A) Schematic representation of ribosome purification procedure.

(B) Immunoblot of the subcellular fractions from WT MEF lysate and adult WT mouse retina lysate.

(C) Immunoblot of the total and purified ribosomal fractions of MEF lysate with increasing KCl concentrations.

(D) Immunoblot of the total and purified ribosomal fractions of MEF lysate, following RNase I treatment of the post-mitochondrial fraction.

(E) Representative polysome profile of WT MEFs on a 15%–50% sucrose gradient. Immunoblot showing the presence of HTT in the 40S, 60S, monosome, and light polysome fractions.

(F) Schematic representation of PLA experiment. PLA staining confocal images on retina sections, showing interaction between HTT and RPS6 or rRNA in RGCs.

(G) PLA staining confocal images showing interaction between HTT and RPS6 or rRNA in WT retinas, and controls with individual antibodies alone.